Polymerase Chain Reaction (PCR):  Steps, Types, Applications | Microbe Online

PCR: Steps Involved in Polymerase Chain Reaction

Polymerase Chain Reaction Overview and Applications

Video: PCR: Principle, Instrumentation, and Applications

Rashi Gupta | PhD Scholar on Instagram: "“Polymerase Chain Reaction (PCR) relies on three fundamental steps—denaturation, annealing, and extension🧬—to achieve precise and exponential amplification of a target DNA sequence. During the denaturation phase (94–98°C), the double-stranded DNA unwinds and separates into single strands by breaking hydrogen bonds, preparing the template for amplification. In the annealing step (50–65°C), the temperature is lowered to allow sequence-specific primers to h